Exodus 4:9 And if they will not believe even these two signs or listen to your voice, then you shall take some of the water of the River and shall take some of the water of the River and pour it on the dry ground, and the water that you take out of the River will become blood upon the dry ground.
The meaning of the third sign, the sign of the water becoming blood, is that in the eyes of God all the earthly supply and worldly enjoyment (the water of the Nile) are nothing but death (blood). The water of the Nile represents our earthly dependency and enjoyment of
worldly and earthly supply. All the supply, enjoyment and entertainment
are death. In order to realize this, we need to pour the water of the Nile upon
the dry land. Dry land being the source of from which life is produced (Gen 1). When they are poured out on that which produces life (the ground), immediately the death is exposed.
Exodus 78:44 And He turned their rivers into blood, As well as their streams, so that they could not drink.
Exodus 105:29 He turned their waters into blood, and killed their fish.
The very supply of that the world affords us is death. To the people of the world, the water in the Nile is wonderful, it irrigates the land of Egypt. However, the called one must see what the water really is.